ART IMAGING TEST MALARIA PARASITES IN HUMAN RED BLOOD CELLS USING IMMERSION OF RAW VEGETABLE OIL WALNUTS (CANARIUMCOMMUNE/C AVENUE) AS AN ALTERNATIVE STANDARD OIL IMMERSION (SYNTHESIS) ON THE OPTICAL MICROSCOPE

  • Zakarias A. Mautuka Chemical Studies Program - Faculty - Kalabahi UNTRIB
  • Martasiana Karbeka Chemical Studies Program - Faculty - Kalabahi UNTRIB
  • Faryda V . Lamma Koly Chemical Studies Program - Faculty - Kalabahi UNTRIB
  • Seprianus Lau Serang Chemical Studies Program - Faculty - Kalabahi UNTRIB
Keywords: Walnut Oil (Canarium commune/C Avenue), oil immersion, Optical Microscopy, Malaria Parasite

Abstract

Malaria Parasites art imaging test results in human red blood cells using immersion oils from plant materials Walnuts (Canarium commune/C Avenue) as an Alternative to Standard Oil Immersion (Synthesis) to the Optical Microscope, has been carried out and the results; Walnuts oil immersion oil can be an alternative to the Optical Microscope. These results are based on three parameters; 1). Photograph art imaging test results and the Standard Oil Immersion Oil looks no different Walnuts nayata, 2). The results of measurements of chemical physics parameters (density, viscosity, refractive index, Num. Acid) both types of oil is slightly different, but the results were not significantly different test, 3). Results Characterization there are similarities wavelengths (short) at 216 nm for Oil Walnuts, and 282 nm for Oil Immersion Standards, both containing similarities functional groups are groups Esters, Carboxylic Acid and Alcohol, and contain unsaturated fatty acids, namely oil immersion Synthesis acidic benzyl Benzoate, and Walnuts immersion oil contains Oleic acid, but for immersion oil Walnuts are also saturated fatty acids namely Palmitate acid, and stearic. Walnut oil is obtained by the method soxhletation, before tested, purified by several methods, namely; Degumisasi, Netralilasi, Bleaching, centrifugation, and Stearin. Immersion oil is needed in the medical world because it is used to identify a wide range of microbiological samples such as Malaria Parasites in Red Blood Cells and Bacteria Tuberculosis in Human Phlegm, if, without the use of oil immersion, the imaging results appear ambiguous and difficult to identify.

Published
2018-06-12